Structural physiology of mitochondria in aging-related diseases Following global trends, Taiwan is on course to becoming an aged society. Mitochondrial dysfunction contributes strongly to aging and aging related-disorders. Mitochondria produce ATPs, lipids and many essential biomolecules; its function is closely associated with its unique cristae-rich double membrane morphology. Elegant partition of membranes and distinctive organization of molecules and machineries allow numerous functions to be executed in dedicated compartments. We aim to characterize 3D architecture and molecular organization in relation to mitochondrial homeostasis and aging. We approach it by electron tomography, correlative light and electron microscopy, and immuno-electron microscopy using Drosophila, mouse and cell culture models. The studies will elucidate how the interplays between architecture and underlie molecular composition contribute to mitochondrial homeostasis and aging.
Structural basis of therapeutic antibodies in targeted therapy of Dengue, Influenza and cancers Therapeutic antibody is an important strategy to tackle viral diseases and cancers. Antibodies function by recognizing viral antigens and neutralizing the infection or by recognizing cancer cell surface antigens allowing targeted therapy. We aim to determine antibody-antigen complex structures by single particle cryo-EM and negative stain reconstruction. We hope to develop curative treatments for Dengue, Influenza infection as well as breast cancer.